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Image Search Results
Journal: Journal of Clinical Microbiology
Article Title: Improved Method for Rapid and Efficient Determination of Genome Replication and Protein Expression of Clinical Hepatitis B Virus Isolates
doi: 10.1128/jcm.02340-10
Figure Lengend Snippet: FIG. 5. Genome replication, virion secretion, and protein expression of PCR clones derived from two thermophilic DNA polymerases. The 4B genome was amplified by 30 cycles of PCR using either High Fidelityplus DNA polymerase or Phusion DNA polymerase and then cloned. The PCR clones were digested with BspQI and further treated with T4 DNA ligase before transfection to Huh7 cells. The uncut original EcoRI dimer of clone 4B served as a positive control for transfection. (A) A fraction of the purified DNA prior to transfection, with the first lane being HindIII-digested DNA. (D) Intracellular envelope proteins were detected by a monoclonal preS2 antibody (specific for the L and M proteins) and a polyclonal S antibody from Novus (less efficient at detecting L and M proteins) or just by the S antibody.
Article Snippet: Virus particles were immunoprecipitated from culture supernatant with a
Techniques: Expressing, Clone Assay, Derivative Assay, Transfection, Positive Control